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In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

In Vivo Imaging of Reactive Oxygen Species in a Murine Wound Model

DOI:
10.3791/58450-v

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06:40 min

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November 17, 2018

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, , , , ,
1Hansjörg Wyss Department of Plastic Surgery,New York University School of Medicine

Chapters

  • 00:04Title
  • 00:36Murine Excisional Wound Healing Model Preparation (Day 0)
  • 01:51Keap1 siRNA (Day 1) and L-012 Solution Preparation and In Vivo Diabetic Wound Imaging (Day 3)
  • 04:01Results: Representative Reactive Oxygen Species (ROS) Quantification and Analysis
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a non-invasive in vivo imaging protocol that is streamlined and cost-effective, utilizing L-012, a chemiluminescent luminol-analog, to visualize and quantify reactive oxygen species (ROS) generated in a mouse excisional wound model.

Tags

Keywords: In Vivo ImagingReactive Oxygen SpeciesMurine Wound ModelRegenerationCytoprotective PathwaysOxidative StressWound HealingDiabetic MiceExcisional WoundBioluminescence ImagingL-012 SolutionKeap1 SiRNA

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