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Journal / Biochemistry / Storproduktion af rekombinant RNA'er på en…
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Biochemistry

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JoVE Journal Biochemistry
Storproduktion af rekombinant RNA'er på en cirkulær stillads ved hjælp af en Viroid-afledte System i Escherichia coli
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Storproduktion af rekombinant RNA'er på en cirkulær stillads ved hjælp af en Viroid-afledte System i Escherichia coli

Article DOI: 10.3791/58472-v • 10:38 min • November 30th, 2018
November 30th, 2018 •
1, 1, 1
1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Spain

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Vi præsenterer her, en protokol til at producere store mængder af rekombinant RNA i Escherichia coli co udtrykke en kimære RNA, der indeholder RNA interesse i en viroid stillads og en plante tRNA ligase. Det vigtigste produkt er en cirkulær molekyle, der letter rensning til ensartethed.

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Biokemi sag 141 rekombinant RNA cirkulært RNA viroid tRNA ligase RNA aptamer Escherichia coli
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