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Journal / Engineering / Etikett-fri Imaging av enkelt proteiner skilles…
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Engineering

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JoVE Journal Engineering
Etikett-fri Imaging av enkelt proteiner skilles ut fra levende celler via iSCAT mikroskopi
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Etikett-fri Imaging av enkelt proteiner skilles ut fra levende celler via iSCAT mikroskopi

Article DOI: 10.3791/58486-v • 10:55 min • November 20th, 2018
November 20th, 2018 •
1, 1,2, 1,3, 4, 4, 1,3
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning, Milligan College, 3Department of Physics, Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology, Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen

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Vi presenterer en protokoll for sanntids optisk påvisning av ett umerket proteiner som de utskilles fra levende celler. Dette er basert på interferometric spredning (iSCAT) mikroskopi, som kan brukes til en rekke ulike biologiske systemer og konfigurasjoner.

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Engineering problemet 141 iSCAT label-fri enkelt-protein mobilnettet sekresjon bildebehandling spredning dynamikk sanntid
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