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通过 iscat 显微镜对活细胞分泌的单一蛋白质进行无标签成像
JoVE Journal
Engineering
This content is Free Access.
JoVE Journal
Engineering
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
通过 iscat 显微镜对活细胞分泌的单一蛋白质进行无标签成像
DOI:
10.3791/58486-v
•
10:55 min
•
November 20, 2018
•
André Gemeinhardt
,
Matthew P. McDonald
2
,
Katharina König
3
,
Michael Aigner
,
Andreas Mackensen
,
Vahid Sandoghdar
3
1
Max Planck Institute for the Science of Light (MPL)
,
2
Area of Scientific Learning
,
Milligan College
,
3
Department of Physics
,
Friedrich Alexander University Erlangen-Nuremberg
,
4
Department of Internal Medicine 5, Hematology and Oncology
,
Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Chapters
00:04
Title
01:18
Building the Interferometric Scattering (iSCAT) Microscope
06:48
iSCAT Microscopy of Secreting Cells
08:53
Results: Label-Free Detection of Proteins Secreted by a Single Cell
09:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种实时光学检测单个未标记蛋白质的协议, 因为它们是从活细胞分泌的。这是基于干涉散射 (iscat) 显微镜, 可应用于各种不同的生物系统和配置。
Tags
Label-free Imaging
Single Proteins
ISCAT Microscopy
Biological Nanoparticles
Extinction Cross Section
Scattering Background
Home-built Microscope
LUZ Cells
Molecular-level Probing
Optical Setup
Damped Optical Table
High Numerical Aperture Objective
Diode Laser
Wide-field Lens
Immersion Oil
Glass Coverslip
Anti-reflection Coated Beamsplitter
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