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JoVE Journal
Engineering

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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
 

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

Article doi: 10.3791/58486
November 20th, 2018

Summary November 20th, 2018

We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.

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