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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
This content is Free Access.
1Max Planck Institute for the Science of Light (MPL), 2Area of Scientific Learning,Milligan College, 3Department of Physics,Friedrich Alexander University Erlangen-Nuremberg, 4Department of Internal Medicine 5, Hematology and Oncology,Friedrich Alexander University Erlangen-Nuremberg (FAU), University Hospital Erlangen
Chapters
- 00:04Title
- 01:18Building the Interferometric Scattering (iSCAT) Microscope
- 06:48iSCAT Microscopy of Secreting Cells
- 08:53Results: Label-Free Detection of Proteins Secreted by a Single Cell
- 09:36Conclusion
We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.
Tags
Keywords: Label-free ImagingSingle ProteinsISCAT MicroscopyBiological NanoparticlesExtinction Cross SectionScattering BackgroundHome-built MicroscopeLUZ CellsMolecular-level ProbingOptical SetupDamped Optical TableHigh Numerical Aperture ObjectiveDiode LaserWide-field LensImmersion OilGlass CoverslipAnti-reflection Coated Beamsplitter
