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Journal
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Environment
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循环介导等温扩增 (灯管) 法快速识别烟粉
虱
JoVE Journal
Environment
This content is Free Access.
JoVE Journal
Environment
A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of
Bemisia tabaci
Please note that all translations are automatically generated.
Click here for the English version.
循环介导等温扩增 (灯管) 法快速识别烟粉
虱
DOI:
10.3791/58502-v
•
05:03 min
•
October 29, 2018
•
Simon Blaser
2,3
,
Hanspeter Diem
,
Andreas von Felten
,
Morgan Gueuning
,
Michael Andreou
,
Neil Boonham
7
,
Jennifer Tomlinson
,
Pie Müller
3
,
Jürg Utzinger
3
,
Beatrice Frey
,
Jürg E. Frey
,
Andreas Bühlmann
1
Department of Method Development and Analytics
,
Agroscope
,
2
Swiss Tropical and Public Health Institute
,
3
University of Basel
,
4
Swiss Federal Plant Protection Service
,
Federal Office for Agriculture
,
5
OptiGene Limited
,
6
Fera Science Limited
,
7
School of Natural and Environmental Sciences
,
Newcastle University
,
8
Department of Plants and Plant Products
,
Agroscope
Chapters
00:04
Title
00:41
Preparing Ready-to-Use
B. tabaci
LAMP Kit
01:39
DNA Extraction and
B. tabaci
LAMP Assay
02:58
LAMP Assay Read-out
03:25
Results: Intercepted
B. tabaci
Insect Specimens Analyzed and Validated via LAMP Assay
04:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文报道了一种基于环路介导等温扩增 (灯管) 技术的
烟粉虱
快速鉴定方法。该议定书要求进行最少的实验室培训, 因此可以在港口和机场等植物进口点现场实施。
Tags
LAMP Assay
Bemisia Tabaci
Insect Pest
DNA Extraction
Isothermal Amplification
LAMP Analysis Device
Melting Temperature Analysis
Automated LAMP Validation
Article
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