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Bioengineering
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一种用于生物技术相关金黄色
葡萄球菌
感染活体可视化和内膜分析的斑马鱼胚胎模型
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated
Staphylococcus aureus
Infection
Please note that all translations are automatically generated.
Click here for the English version.
一种用于生物技术相关金黄色
葡萄球菌
感染活体可视化和内膜分析的斑马鱼胚胎模型
DOI:
10.3791/58523-v
•
10:04 min
•
January 07, 2019
•
Xiaolin Zhang
2
,
Leonie de Boer
,
Oliver W. Stockhammer
,
Dirk W. Grijpma
3
,
Herman P. Spaink
,
Sebastian A.J. Zaat
1
Department of Medical Microbiology
,
Amsterdam UMC
,
2
Technical Medical Center, Department of Biomaterials Science and Technology
,
University of Twente
,
3
Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen
,
University of Groningen
,
4
Institute of Biology
,
Leiden University
Chapters
00:04
Title
00:44
“Bacteria-only” and Bacteria-microspheres Suspension Preparation
03:04
Zebrafish Embryo Injection
05:50
Bacterial Culture Quantification and Infection Progression Monitoring
07:37
Results: Representative Intravital Biomaterial-associated
Staphylococcus aureus
Infection Analysis
09:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究描述了一种斑马鱼胚胎模型, 用于基于荧光显微镜的生物物质相关感染的体内可视化和体内分析。该模型是一个很有前途的系统, 补充哺乳动物动物模型, 如研究体内生物材料相关感染的小鼠模型。
Tags
Zebrafish Embryo
In Vivo Visualization
Intravital Analysis
Biomaterial-associated Infection
Staphylococcus Aureus
Bacteria-only Suspension
Bacteria Microsphere Suspension
Polystyrene Microspheres
Fluorescent Staph Aureus MCherry Strain
Colony Counting
Optical Density
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