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体外
培养肺泡上皮细胞中卤代气体浓度的控制方法
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Medicine
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JoVE Journal
Medicine
In Vitro
Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
体外
培养肺泡上皮细胞中卤代气体浓度的控制方法
DOI:
10.3791/58554-v
•
04:56 min
•
October 23, 2018
•
Raïko Blondonnet
2
,
Bertille Paquette
2
,
Damien Richard
,
Rémi Bourg
4
,
Géraldine Laplace
4
,
Romain Segurel
4
,
Henria Pouvelle
4
,
Corinne Belville
,
Loic Blanchon
,
Thomas Godet
2
,
Jean-Michel Constantin
2
,
Jean-Etienne Bazin
2
,
Vincent Sapin
,
Matthieu Jabaudon
2
1
Department of Perioperative Medicine
,
CHU Clermont-Ferrand
,
2
Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD)
,
Université Clermont Auvergne
,
3
Department of Pharmacology
,
CHU Clermont-Ferrand
,
4
Nurse Anesthetist School
,
CHU Clermont-Ferrand
Chapters
00:04
Title
00:40
Preparation of an Air-tight Chamber
01:57
Expose Alveolar Epithelial Cells to Halogenated Agents
03:13
Results: Concentration of Sevoflurane and Isoflurane Over Time
03:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一个简单的协议, 专门设计, 以达到精确和控制的浓度七氟醚或异氟醚
在体外
, 以提高我们对介入的机制在上皮性肺损伤的理解和测试小说急性呼吸窘迫综合征的治疗。
Tags
In Vitro
Halogenated Gases
Alveolar Epithelial Cells
Lung Injury
Acute Respiratory Distress Syndrome
Airtight Chamber
Gas Flow
Carbon Dioxide
Sevoflurane
Isoflurane
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