Journal
/
/
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
DOI:

04:56 min

October 23, 2018

, , , , , , , , , , , , ,

Chapters

  • 00:04Title
  • 00:40Preparation of an Air-tight Chamber
  • 01:57Expose Alveolar Epithelial Cells to Halogenated Agents
  • 03:13Results: Concentration of Sevoflurane and Isoflurane Over Time
  • 03:56Conclusion

Summary

Automatic Translation

We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.

Related Videos

Read Article