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In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
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JoVE Journal Medicine
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

DOI:
10.3791/58554-v

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04:56 min

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October 23, 2018

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, , , , , , , , , , , , ,
1Department of Perioperative Medicine,CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD),Université Clermont Auvergne, 3Department of Pharmacology,CHU Clermont-Ferrand, 4Nurse Anesthetist School,CHU Clermont-Ferrand

Chapters

  • 00:04Title
  • 00:40Preparation of an Air-tight Chamber
  • 01:57Expose Alveolar Epithelial Cells to Halogenated Agents
  • 03:13Results: Concentration of Sevoflurane and Isoflurane Over Time
  • 03:56Conclusion

Summary

Automatic Translation

Automatic Translation

We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.

Tags

In VitroHalogenated GasesAlveolar Epithelial CellsLung InjuryAcute Respiratory Distress SyndromeAirtight ChamberGas FlowCarbon DioxideSevofluraneIsoflurane

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