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Påvisning av Tilapia Lake Virus bruker konvensjonelle RT PCR og SYBR grønne RT-qPCR
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Påvisning av Tilapia Lake Virus bruker konvensjonelle RT PCR og SYBR grønne RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

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Denne protokollen diagnostiserer Tilapia Lake Virus (TiLV) i tilapia vev bruker RT PCR metoder. Hele metoden er beskrevet fra vev disseksjon til totale RNA utvinning, etterfulgt av cDNA syntese og oppdagelse av TiLV ved konvensjonelle PCR eller kvantitativ PCR bruker dsDNA bindende en fluorescerende bindende fargestoff.

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Miljøfag problemet 141 Tilapia innsjøen virus tilapia diagnose RT PCR RT-qPCR orthomyxo-lignende virus
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