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Engineering
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ワンステップ低温大気プラズマ治療
体外
で神経幹細胞の分化
JoVE Journal
Engineering
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JoVE Journal
Engineering
Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
ワンステップ低温大気プラズマ治療
体外
で神経幹細胞の分化
DOI:
10.3791/58663-v
•
07:01 min
•
January 11, 2019
•
Zilan Xiong*
1
,
Shasha Zhao*
2
,
Xu Yan
1
State Key Laboratory of Advanced Electromagnetic Engineering and Technology
,
Huazhong University of Science and Technology
,
2
College of Life Science and Health
,
Wuhan University of Science and Technology
,
3
Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital
,
Capital Medical University
Chapters
00:04
Title
00:44
Murine Neural Stem-Cell (NSC) Culture and Pre-Differentiation
02:02
Jet Acquisition
02:57
NSC Plasma Treatment and Differentiation
04:12
Immunofluorescence Staining
05:35
Results: Representative NSC Fate Specification
06:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、神経幹細胞と分化強化のため蛍光検出に冷たい大気圧プラズマ処理の詳細な実験手順を提供しています。
Tags
Neural Stem Cells
Cold Atmospheric Plasma
Differentiation
Parkinson’s Disease
Murine Neural Stem Cells
DMEM
Poly-D-lysine
Differentiation Medium
Plasma Jet
Helium
Oxygen
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