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Biochemistry

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How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
 

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Article DOI: 10.3791/58588-v
January 7th, 2019

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Summary January 7th, 2019

Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.

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