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Biochemistry
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如何稳定蛋白质: 病毒 x 项目中热移检测和纳米差动扫描荧光测量的稳定性筛选
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Please note that all translations are automatically generated.
Click here for the English version.
如何稳定蛋白质: 病毒 x 项目中热移检测和纳米差动扫描荧光测量的稳定性筛选
DOI:
10.3791/58666-v
•
07:22 min
•
February 11, 2019
•
Daniel Bruce
,
Emily Cardew
,
Stefanie Freitag-Pohl
,
Ehmke Pohl
2
1
Department of Biosciences
,
Durham University
,
2
Department of Chemistry
,
Durham University
Chapters
00:04
Title
00:27
Preparation of Protein Sample
01:31
Performing a Thermal Shift Assay (TSA) Experiment
03:22
Preparing and Programing a Nano Differential Scanning Fluorimetry (nanoDSF) Experiment
05:35
Results: Analysis of the Temperature at the Inflection Point of the Unfolding Transition (T
m
) in Lysozyme
06:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了一种通过热移分析和纳米差动扫描荧光法快速测试蛋白质在各种条件下的热稳定性的方案。缓冲系统、盐和添加剂, 包括三个独特的稳定屏幕, 与蛋白质一起进行检测, 以确定适合功能和结构研究的缓冲液。
Tags
Protein Stabilization
Thermal Shift Assay
Nano Differential Scanning Fluorimetry
Protein Chemistry
Drug Discovery
Stability Screen
Protein Thermostability
Protein Ligand Binding
High-throughput
SYPRO Orange Dye
RT-PCR System
Melt Curve Analysis
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