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Biochemistry
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利用高含量成像对增强细胞中的目标参与进行量化
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
Please note that all translations are automatically generated.
Click here for the English version.
利用高含量成像对增强细胞中的目标参与进行量化
DOI:
10.3791/58670-v
•
07:23 min
•
November 29, 2018
•
Hanna Axelsson
2
,
Helena Almqvist
2
,
Brinton Seashore-Ludlow
3
1
Chemical Biology Consortium Sweden, Science for Life Laboratory
,
Karolinska Institutet, Solna
,
2
Department of Medical Biochemistry and Biophysics
,
Karolinska Institutet, Solna
,
3
Science for Life Laboratory, Department of Oncology-Pathology
,
Karolinska Institutet, Stockholm
Chapters
00:04
Title
00:31
Cell Seeding
01:51
Compound Treatment and Heat Challenge
03:30
Staining, Image Acquisition and Analysis
05:33
Results: Representative Target Engagement Quantification by High Content Imaging
06:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
药物靶向接触的测量是有效药物开发和化学探针验证的核心。在这里, 我们详细介绍了一种在细胞热转移分析 (cetsa) 的微板兼容适应中使用高含量成像测量药物靶点接触的协议。
Tags
High Content Imaging
Target Engagement
Adherent Cells
Chemical Biology
Drug Discovery
Cell Lines
Primary Cultures
Cocultures
A-431 Cells
Trypsin
Plate Washing
Compound Treatment
Heat Challenge
Water Bath
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