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利用高含量成像对增强细胞中的目标参与进行量化
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
DOI:

07:23 min

November 29, 2018

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Chapters

  • 00:04Title
  • 00:31Cell Seeding
  • 01:51Compound Treatment and Heat Challenge
  • 03:30Staining, Image Acquisition and Analysis
  • 05:33Results: Representative Target Engagement Quantification by High Content Imaging
  • 06:54Conclusion

Summary

Automatic Translation

药物靶向接触的测量是有效药物开发和化学探针验证的核心。在这里, 我们详细介绍了一种在细胞热转移分析 (cetsa) 的微板兼容适应中使用高含量成像测量药物靶点接触的协议。

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