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Genetics

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Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
 

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Article doi: 10.3791/58682
November 21st, 2018

Summary November 21st, 2018

Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.

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