Journal
/
Genetics
/
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

DOI:
10.3791/58682-v

•

07:50 min

•

November 21, 2018

•

, , ,
1Biochemistry Department,Boston University School of Medicine, 2Bioinformatic Hub,Boston University

Chapters

  • 00:04Title
  • 00:34Dissection and Preparation of BAT for Chromatin Immunoprecipitation
  • 03:29Immune Complexes Collection and DNA Recovery
  • 05:51Results: ChIP Validation by qPCR
  • 07:17Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.

Tags

Keywords: Chromatin ImmunoprecipitationChIPMouseFormaldehyde Cross-linkingCell LysisSonicationImmunoprecipitationAntibodyProtein A Agarose

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58682?article_type=v

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
check_url/58682?article_type=v

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
check_url/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
check_url/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
check_url/58682?article_type=v

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
check_url/58682?article_type=v

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
check_url/58682?article_type=v

High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
check_url/58682?article_type=v

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications
check_url/58682?article_type=v

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Read Article