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小鼠棕色脂肪组织的染色质免疫沉淀
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
DOI:

07:50 min

November 21, 2018

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Chapters

  • 00:04Title
  • 00:34Dissection and Preparation of BAT for Chromatin Immunoprecipitation
  • 03:29Immune Complexes Collection and DNA Recovery
  • 05:51Results: ChIP Validation by qPCR
  • 07:17Conclusion

Summary

Automatic Translation

在这里, 我们描述了有效染色质免疫沉淀 (chip) 的协议, 然后是从小鼠身上分离出的棕色脂肪组织 (bat) 的高通量 dna 测序 (chip-seq)。该方案既适用于组蛋白修饰的映射, 也适用于研究感兴趣的非组蛋白在体内的全基因组定位。

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