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A Rhodopsin Transport Assay by High-Content Imaging Analysis
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JoVE Journal Chemistry
A Rhodopsin Transport Assay by High-Content Imaging Analysis

A Rhodopsin Transport Assay by High-Content Imaging Analysis

DOI:
10.3791/58703-v

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12:11 min

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January 16, 2019

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, ,
1Department of Ophthalmology,University of Pittsburgh, 2McGowan Institute for Regenerative Medicine,University of Pittsburgh

Chapters

  • 00:04Title
  • 01:19Treating the Cells with Compounds
  • 02:19Immunostaining without Membrane Permeabilization to Stain Rhodopsin Protein on the Cell Surface
  • 04:43Imaging
  • 07:12Image Analysis
  • 09:12Results: Characterization of Rhodopsin Transport
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we described a high-content imaging method to quantify the transport of rhodopsin mutants associated with retinitis pigmentosa. A multiple-wavelength scoring analysis was used to quantify rhodopsin protein on the cell surface or in the whole cell.

Tags

Keywords: Rhodopsin TransportRetinitis PigmentosaHigh-content Imaging AnalysisMembrane Protein Localization384-well Plate AssayImmunostainingDrug ScreeningZ-factor9-cis-retinal

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