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Journal
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Chemistry
/
高含量成像分析研究。
JoVE Journal
Chemistry
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JoVE Journal
Chemistry
A Rhodopsin Transport Assay by High-Content Imaging Analysis
Please note that all translations are automatically generated.
Click here for the English version.
高含量成像分析研究。
DOI:
10.3791/58703-v
•
12:11 min
•
January 16, 2019
•
Bing Feng
,
Xujie Liu
,
Yuanyuan Chen
2
1
Department of Ophthalmology
,
University of Pittsburgh
,
2
McGowan Institute for Regenerative Medicine
,
University of Pittsburgh
Chapters
00:04
Title
01:19
Treating the Cells with Compounds
02:19
Immunostaining without Membrane Permeabilization to Stain Rhodopsin Protein on the Cell Surface
04:43
Imaging
07:12
Image Analysis
09:12
Results: Characterization of Rhodopsin Transport
10:50
Conclusion
Summary
Automatic Translation
English (Original)
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在这里, 我们描述了一种高含量的成像方法来量化与视网膜色素变性相关的视紫红质突变体的转运。采用多波长评分分析法对细胞表面或整个细胞的视紫红质蛋白进行了定量。
Tags
Rhodopsin Transport
Retinitis Pigmentosa
High-content Imaging Analysis
Membrane Protein Localization
384-well Plate Assay
Immunostaining
Drug Screening
Z-factor
9-cis-retinal
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