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JoVE Journal Chemistry
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En Rhodopsin Transport test av hög-innehåll Imaging analys

Article DOI: 10.3791/58703-v • 12:11 min • January 16th, 2019
January 16th, 2019 •
1, 1, 1,2
1Department of Ophthalmology, University of Pittsburgh, 2McGowan Institute for Regenerative Medicine, University of Pittsburgh

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Vi beskrivit här, en hög-innehåll bildgivande metod för att kvantifiera transport av rhodopsin mutanter är associerad med retinitis pigmentosa. En multipel-våglängd scoring analys användes för att kvantifiera rhodopsin protein på cellens yta eller i hela cellen.

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Kemi fråga 143 hög-innehåll imaging felveckning rhodopsin farmakologiska förkläde retinitis pigmentosa protein immunfärgning
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