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Journal / Biology / Droplet stregkodesystem-baserede enkelt celle…
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Biology

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JoVE Journal Biology
Droplet stregkodesystem-baserede enkelt celle Transcriptomics af voksne pattedyr væv
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Droplet stregkodesystem-baserede enkelt celle Transcriptomics af voksne pattedyr væv

Article DOI: 10.3791/58709-v • 10:12 min • January 10th, 2019
January 10th, 2019 •
*1,2,3, *2, 2, 2, 1,4, 2, 1,4, 1,2,3
1Hotchkiss Brain Institute, University of Calgary, 2Department of Comparative Biology and Experimental Medicine, Faculty of Veterinary Medicine, University of Calgary, 3Alberta Children's Hospital Research Institute, University of Calgary, 4Department of Clinical Neurosciences, Cumming School of Medicine, University of Calgary
* These authors contributed equally

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Denne protokol beskriver de generelle processer og kvalitetskontrol kontrol nødvendig for at forberede droplet-baseret, høj overførselshastighed enkelt celle RNA-Seq præparater sunde voksne pattedyr enkelt celler. Sekventering parametre, tilbydes Læs justering og downstream encellede bioinformatic analyse også.

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Biologi spørgsmålet 143 enkelt celle RNA-FF. voksen transkriptom RNA FACS
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