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JoVE Journal Biochemistry
De structuur en de dynamiek van Nucleosomes met behulp van Atomic Force microscopie Imaging sonderen
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De structuur en de dynamiek van Nucleosomes met behulp van Atomic Force microscopie Imaging sonderen

Article DOI: 10.3791/58820-v • 09:52 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1
1Department of Pharmaceutical Sciences, University of Nebraska Medical Center

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Hier presenteren we een protocol karakteriseren nucleosoom deeltjes op het niveau van de single-molecuul met behulp van statische en time-lapse atomaire kracht microscopie (AFM) beeldvormende technieken. De beschreven methode van oppervlakte functionalization zorgt voor de opname van de structuur en de dynamiek van nucleosomes in hoge resolutie op nanoschaal.

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Biochemie kwestie 143 AFM time-lapse nucleosoom chromatine dynamica structuur histonen single - molecuul DNA epigenetica imaging
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