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Sondering struktur og dynamik af Nucleosomes ved hjælp af Atomic Force mikroskopi Imaging
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
DOI:

09:52 min

January 31, 2019

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Chapters

  • 00:04Title
  • 01:06Functionalization of Mica Surface for Static AFM Imaging of Nucleosomes
  • 02:37Preparation of Nucleosome Samples on APS-Mica for Static AFM Imaging
  • 03:39Static AFM Imaging of Nucleosomes
  • 04:42High-Speed Time-Lapse AFM Imaging of Nucleosome Dynamics
  • 07:42Results: Probing the Structure and Dynamics of Nucleosomes
  • 09:29Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol til at karakterisere nucleosome partikler på enkelt-molekyle niveau ved hjælp af statisk og time-lapse atomic force mikroskopi (AFM) imaging teknikker. Den beskrevne overflade functionalization metode giver mulighed for opsamling af strukturen og dynamikken i nucleosomes i høj opløsning på nanoplan.

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