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Immunology and Infection
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尿道感染小鼠模型产生的单细胞内细菌群落的分离, 用于下游单细胞分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
Please note that all translations are automatically generated.
Click here for the English version.
尿道感染小鼠模型产生的单细胞内细菌群落的分离, 用于下游单细胞分析
DOI:
10.3791/58829-v
•
07:34 min
•
April 16, 2019
•
EnJun Yang
,
Jacqueline L.Y. Chee
,
Suhanya Duraiswamy
,
Siyi Chen
,
Kristin Lees
,
Swaine L. Chen
3
1
Infectious Diseases Group
,
Genome Institute of Singapore
,
2
Department of Chemical and Biomolecular Engineering
,
National University of Singapore
,
3
Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine
,
National University of Singapore
Chapters
00:04
Title
00:41
Glass Capillary Preparation
02:06
Bladder Epithelial Cell Harvest
03:53
Intracellular Bacterial Community (IBC) Isolation
05:18
Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
06:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了一种从尿路感染的小鼠模型中分离单个感染膀胱上皮细胞的简单方法。
Tags
Intracellular Bacterial Communities
IBCs
Urinary Tract Infection
UTI
Mouse Bladder
Mouth Micropipetting
Glass Capillary Tube
Bladder Epithelial Cells
Single-cell Analysis
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