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Immunology and Infection
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Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

DOI:
10.3791/54432-v

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10:23 min

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December 01, 2017

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, ,
1Unité d’Immunobiologie des Cellules Dendritiques, Department of Immunology,Institut Pasteur, INSERM U818, 2Genentech

Chapters

  • 00:05Title
  • 00:47Preparation of Catheters and Uropathogenic E. coli for Infection
  • 02:08Transurethral Instillation of Female Mice
  • 03:43Transurethral Instillation of Male Mice
  • 04:56Determination of CFU in Infected Organs
  • 06:09Flow Cytometric Analysis of Bladder Tissue
  • 08:31Results: Transurethral Catheterization to Induce Urinary Tract Infections
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

The established model of transurethral catheterization of mice allows the study of bladder pathologies, including urinary tract infection, but can only be performed in females. A new model of male transurethral instillation, presented here, will enable research in an area marked by strong clinical and epidemiological differences between the sexes.

Tags

Keywords: Urinary Tract InfectionSmall Animal ModelTransurethral CatheterizationMale And Female MiceMucosal ImmunologyUropathogenic E. ColiInoculumBladder Instillation

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