JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
下流の単一細胞分析のため尿路感染症のマウスモデルから生成された単一細胞内細菌群集の分離
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
Please note that all translations are automatically generated.
Click here for the English version.
下流の単一細胞分析のため尿路感染症のマウスモデルから生成された単一細胞内細菌群集の分離
DOI:
10.3791/58829-v
•
07:34 min
•
April 16, 2019
•
EnJun Yang
,
Jacqueline L.Y. Chee
,
Suhanya Duraiswamy
,
Siyi Chen
,
Kristin Lees
,
Swaine L. Chen
3
1
Infectious Diseases Group
,
Genome Institute of Singapore
,
2
Department of Chemical and Biomolecular Engineering
,
National University of Singapore
,
3
Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine
,
National University of Singapore
Chapters
00:04
Title
00:41
Glass Capillary Preparation
02:06
Bladder Epithelial Cell Harvest
03:53
Intracellular Bacterial Community (IBC) Isolation
05:18
Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
06:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
このプロトコルでは、単純な尿路感染症のマウスモデルからの単一、感染して膀胱の上皮細胞を分離する方法について説明します。
Tags
Intracellular Bacterial Communities
IBCs
Urinary Tract Infection
UTI
Mouse Bladder
Mouth Micropipetting
Glass Capillary Tube
Bladder Epithelial Cells
Single-cell Analysis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
マウス尿路感染症の経尿道的誘導
のRNAの単離
緑膿菌
マウス消化管に定着
細胞内細菌性病原体を持つゼブラフィッシュ胚の感染
の単一細胞解析
枯草菌
バイオフィルム
'シングルウイルスゲノミクス'を使用してシングルビリオンの単離とゲノム解析
マウス生殖管粘膜からのリンパ球の単離
細胞内病原体によって引き起こされる液胞破裂のシングルセル測定
別のマウス皮膚の領域から細胞蛍光分析のための単一細胞懸濁液の調製
小動物モデルにおける尿路感染症: 雌雄マウスの尿道カテーテル
単一細胞の蛍光顕微鏡による Efferocytosis の定量化
Read Article