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Isolering av enkelt intracellulær bakteriell samfunn generert fra en Murine modell av urinveisinfeksjon for nedstrøms encellede analyse
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
DOI:

07:34 min

April 16, 2019

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Chapters

  • 00:04Title
  • 00:41Glass Capillary Preparation
  • 02:06Bladder Epithelial Cell Harvest
  • 03:53Intracellular Bacterial Community (IBC) Isolation
  • 05:18Results: Harvested IBC Are Pure and Can Be Used for Downstream Analysis
  • 06:30Conclusion

Summary

Automatic Translation

Denne protokollen beskriver en enkel metode for å isolere enkelt, infisert-blæren epitelceller fra en murine modell av urinveisinfeksjon.

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