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高通量硝基苯并二氮唑标记的胆固醇积液测定
JoVE Journal
Medicine
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JoVE Journal Medicine
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
DOI:

08:18 min

January 07, 2019

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Chapters

  • 00:04Title
  • 01:05NBD-cholesterol Preparation, Cell Culture, and Seeding
  • 02:20Apolipoprotein B Depleted Serum (ABDS) Preparation
  • 03:14NBD-cholesterol Cell Loading and Incubation with Cholesterol Acceptors
  • 04:29Fluorescent Signal Capture
  • 05:35Results: Analysis of Cholesterol Efflux Assay
  • 07:24Conclusion

Summary

Automatic Translation

在巨噬细胞模型中测量血清或血浆的体外胆固醇流出能力是动脉粥样硬化的一个很有前途的工具。在本研究中, 我们优化和规范了荧光 nbd-胆固醇外排法, 并开发了一个使用96孔板的高通量分析。

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