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3D 扫描技术在 3D 新颖嵌入重叠协议中桥接微电路和宏观大脑图像

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

3D Scanning Technology Bridging Microcircuits and Macroscale Brain Images in 3D Novel Embedding Overlapping Protocol

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3D 扫描技术在 3D 新颖嵌入重叠协议中桥接微电路和宏观大脑图像

DOI:

10.3791/58911-v

•

10:14 min

•

May 12, 2019

•

Saya Ide, Motoki Kajiwara, Hirohiko Imai, Masanori Shimono

¹Graduate School of Medicine and Faculty of Medicine,Kyoto University, ²Graduate School of Informatics,Kyoto University

Chapters

00:04Title
01:09Brain Surface Scan, Slicing, and Multi-electrode Array Recording
05:23MRI Image Processing to Stripe Cortical Surfaces
06:32Preprocessing for 3D Scan Data and Coregistration of the MRI Surface and the 3D Scan Surface
08:18Results: Overlapping Accuracy Between MRIs and 3D Scans
09:17Conclusion

Summary

Automatic Translation

本文介绍了一种利用三维扫描技术将两个空间尺度连接起来的实验方案:由MRI在>100 μm处成像的全脑解剖结构的宏观空间尺度,以及使用神经元分布的微观空间尺度免疫组织化学染色和多电极阵列系统等方法(±10μm)。

Tags

3D Scanning Neuroanatomy Micro-scale Macro-scale Brain Imaging 3D Neuro Embedding MRI Post-mortem Brain Water Sensitivity Sample Preparation Mouse Brain Automatic Turntable 3D Scan Protocol

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