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Un saggio di comunicazione intercellulare Junction ioduro-giallo fluorescente proteina-Gap
JoVE Journal
Biology
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JoVE Journal Biology
An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
DOI:

09:47 min

February 01, 2019

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Chapters

  • 00:04Title
  • 01:05Generation of LN215-YFPQL and LN215-I” Cells by Lentiviral Transduction
  • 03:14Plating the LN215-YFPQL and LN215-I Cells
  • 04:42Conducting the I-YFP GJIC Assay
  • 07:37Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
  • 08:15Conclusion

Summary

Automatic Translation

Qui, presentiamo un protocollo per un dosaggio di comunicazione intercellulare di romanzo gap junction progettato per lo screening ad alta resa di prodotti chimici di gap junction-modulanti per drug discovery e valutazione tossicologica.

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