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一种碘-黄色荧光荧光蛋白-缝隙--细胞间通信方法
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Biology
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JoVE Journal
Biology
An Iodide-Yellow Fluorescent Protein-Gap Junction-Intercellular Communication Assay
Please note that all translations are automatically generated.
Click here for the English version.
一种碘-黄色荧光荧光蛋白-缝隙--细胞间通信方法
DOI:
10.3791/58966-v
•
09:47 min
•
February 01, 2019
•
Joo Hye Yeo
,
Jinu Lee
1
College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences
,
Yonsei University
Chapters
00:04
Title
01:05
Generation of LN215-YFP
QL
and LN215-I” Cells by Lentiviral Transduction
03:14
Plating the LN215-YFP
QL
and LN215-I
‐
Cells
04:42
Conducting the I-YFP GJIC Assay
07:37
Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
08:15
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一种新的缝隙结细胞间通信检测的协议, 旨在为药物发现和毒理学评估的间隙结调节化学品的高通量筛选。
Tags
Iodide-YFP
Gap Junction
Intercellular Communication
High-throughput Screening
Assay
LN215 Cells
Lentivirus
Puromycin
Cell Culture
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