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Journal / Developmental Biology / Effiziente Differenzierung menschlicher…
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Developmental Biology

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JoVE Journal Developmental Biology
Effiziente Differenzierung menschlicher pluripotenter Stammzellen in Leberzellen
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Effiziente Differenzierung menschlicher pluripotenter Stammzellen in Leberzellen

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Dieses Protokoll beschreibt eine monolayer, serumfreie Methode zur effizienten Erzeugung von Hepatozyten-ähnlichen Zellen aus menschlichen pluripotenten Stammzellen (hPSCs) in 18 Tagen. Dies beinhaltet sechs Schritte, da hPSCs sequenziell in zwischengeschaltete Zelltypen wie den Primitivstreifen, das definitive Endoderm, das hintere Vorgut und die Leberknospenvorläufer differenzieren, bevor hepatozytenähnliche Zellen gebildet werden.

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Entwicklungsbiologie Ausgabe 148 Mensch pluripotente Stammzelle effizient Differenzierung Endoderm Leber Vorläufer Hepatozyten
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