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JoVE Journal Developmental Biology
Effektiv differensiering av Human Pluripotent stamceller i leverceller
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Effektiv differensiering av Human Pluripotent stamceller i leverceller

Article DOI: 10.3791/58975-v • 07:37 min • June 11th, 2019
June 11th, 2019 •
1,2, 1, 1
1Institute for Stem Cell Biology & Regenerative Medicine, Stanford-UC Berkeley Siebel Stem Cell Institute, Stanford University School of Medicine, 2Department of Developmental Biology, Stanford University School of Medicine

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Denne protokollen detaljer en monolag, serum-fri metode for å effektivt generere hepatocytter celler fra humant Pluripotent stamceller (hPSCs) i 18 dager. Dette medfører seks trinn som hPSCs sekvensielt differensiere til mellomliggende celle-typer som primitive strek, definitive endoderm, bakre foregut og lever knopp forfedre før forming hepatocytter celler.

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Utviklingsbiologi Human pluripotent stilk cellen effektiv differensiering endoderm lever stamfar hepatocytter
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