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Journal
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Bioengineering
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人原发性前列腺组织培养的处理与评价
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Handling and Assessment of Human Primary Prostate Organoid Culture
Please note that all translations are automatically generated.
Click here for the English version.
人原发性前列腺组织培养的处理与评价
DOI:
10.3791/59051-v
•
08:45 min
•
January 17, 2019
•
Tara McCray*
1
,
Zachary Richards*
1
,
Joseph Marsili
,
Gail S. Prins
2,3
,
Larisa Nonn
3
1
Department of Pathology
,
University of Illinois at Chicago
,
2
Departments of Urology, Physiology, and Biophysics
,
University of Illinois at Chicago
,
3
University of Illinois Cancer Center
Chapters
00:04
Title
01:04
Collection of Organoids from Matrix Gel
02:09
Formalin Fixation and Paraffin Embedding of Organoids for Histological Endpoints
04:00
Sectioning the Formalin Fixed Paraffin Embedded (FFPE) Histology Gel Blocks
05:04
Whole-mounting of Organoids for Immunofluorescent Staining
06:35
Results: Analysis of Human Primary Prostate Organoids
07:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个协议, 以指导人类的原发性前列腺有机体处理, 然后建议终点, 以评估表型。介绍了种子、培养维护、基质凝胶回收、形态定量、嵌入切片、ffpe 切片、全装染色及商业检测的应用。
Tags
Prostate Organoids
In Vitro Culture
Primary Cells
Matrix Gel
Neutral Protease
Histology Embedding
Precision Medicine
Disease Modeling
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