A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Chemistry Department,Brooklyn College, 2Ph.D. Program in Biochemistry,Graduate Center of the City University of New York, 3Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 4Ph.D. Programs in Chemistry, Biochemistry, and Biology,Graduate Center of the City University of New York
Chapters
- 00:04Title
- 01:00Neurodegenerative Proteinopathy-associated Protein Overexpression in S. cerevisiae
- 03:02Cell Lysis and Protein Transfer
- 05:28Histone Post-translational Modification Detection
- 06:52Results: Representative Effects of Yeast Protein Overexpression on Histone Expression and Modification
- 08:03Conclusion
Questo protocollo delinea le procedure sperimentali per caratterizzare il genoma cambiamenti nei livelli di modifiche post-traduzionali degli istoni (PTM) che si verificano in relazione la sovraespressione di proteine associate con ALS e malattia del Parkinson in Modelli di Saccharomyces cerevisiae . Dopo la separazione di SDS-PAGE, vengono rilevati livelli PTM istone individuali con gli anticorpi specifici di modifiche tramite Western blotting.
