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Biochemistry

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JoVE Journal Biochemistry
Analyse af Endocytic optagelse og retrograd Transport til Trans-Golgi netværket ved hjælp af Functionalized Nanobodies i kulturperler celler
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Analyse af Endocytic optagelse og retrograd Transport til Trans-Golgi netværket ved hjælp af Functionalized Nanobodies i kulturperler celler

Article DOI: 10.3791/59111-v • 11:05 min • February 21st, 2019
February 21st, 2019 •
1, 1
1Biozentrum, University of Basel

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Retrograd transport af proteiner fra cellens overflade til Golgi er afgørende at fastholde membran homøostase. Her, beskriver vi en metode til at analysere biokemisk celle overflade til Golgi transport af rekombinante proteiner ved hjælp af functionalized nanobodies i HeLa celler.

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Immunologi og infektion spørgsmålet 144 nanobodies retrograd transport Golgi kompleks tyrosin sulfation bakteriel udtryk radiolabeling EGFP HeLa celler
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