JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
单链 mhc 技术在人 cd8+ t 细胞活化中的相关研究
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Please note that all translations are automatically generated.
Click here for the English version.
单链 mhc 技术在人 cd8+ t 细胞活化中的相关研究
DOI:
10.3791/59126-v
•
12:09 min
•
February 28, 2019
•
Xiang Zhao
,
Maryam Hamidinia
,
Joanna Ai Ling Choo
,
Chien Tei Too
2
,
Zi Zong Ho
,
Ee Chee Ren
,
Antonio Bertoletti
,
Paul A. MacAry
2,5
,
Keith G. Gould
,
Joanna Brzostek
,
Nicholas R.J. Gascoigne
2,5
1
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine
,
National University of Singapore
,
2
Immunology Programme, Life Sciences Institute
,
National University of Singapore
,
3
Emerging Infectious Diseases Program
,
Duke-NUS Graduate Medical School
,
4
Singapore Immunology Network
,
A*STAR
,
5
NUS Graduate School for Integrative Sciences and Engineering (NGS)
,
National University of Singapore
,
6
Department of Immunology, Wright-Fleming Institute
,
Imperial College London
Chapters
00:04
Title
01:19
Plating CHO Cells as Antigen Presenting Cells
04:03
T Cell Activation Assay
07:52
CHO Cell MHC Class I Staining
09:06
Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
10:46
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了利用单链 mhc i 类复合物研究人类 cd8 + t 细胞激活过程中的分子相互作用: 生成表达单链结构的工程抗原呈现细胞, 培养人类 cd8 + t 细胞克隆和 t 细胞活化实验。
Tags
Single Chain MHC
Co-agonism
Human CD8+ T Cell Activation
Nonstimulatory Peptide MHC
Agonist Peptide MHC
Hepatitis B Virus
HIV Gag
Tetracycline-inducible Expression
CHO Cells
T Cell Activation Experiment
Trypsinization
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
T细胞受体在小鼠T细胞的逆转录病毒转导
使用γ-干扰素酶酶联免疫检测的特点人类乳头状瘤病毒的新型T-细胞表位
移植物抗宿主病的诱导和<em在体内</em> T细胞监测中的应用MHC匹配的小鼠模型
piggyBac转座子
初级人类T细胞系统改造
荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
人全血中NK细胞杀伤活性的流式细胞仪分析
猴免疫缺陷病毒特异性CD8分析<sup> +</sup> T细胞猕猴由肽MHC-I四聚体染色
原位
MHC-聚丙烯染色和定量分析确定抗原特异性 CD8 T 细胞在组织中的位置、丰度和表型
一种研究人体 T 细胞召回反应
的
吸水泡协议
用病毒载体接种后, mhc i 四分体染色同时定量抗载体和抗转基因特异性 cd8
+
t 细胞
Read Article