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Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

DOI:
10.3791/58680-v

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08:10 min

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November 28, 2018

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, , ,
1Division of Virology, Department of Hygiene, Microbiology, Social Medicine,Medical University of Innsbruck

Chapters

  • 00:04Title
  • 00:49Sample Collection
  • 01:21Staining Set-up
  • 01:59Tetramer Staining and Preparation of Antibodies
  • 03:17Staining of Samples
  • 04:24Lysis of Erythrocytes
  • 05:09Flow Cytometric Measurement and Analysis
  • 06:53Results: Quantification of Antigen-specific CD8+ T Cells in Mouse Blood of Lymphoid Organs
  • 07:36Conclusion

Summary

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Automatic Translation

Here, we present a protocol for the ex vivo qualitative detection of antigen-specific CD8+ T cells. Analysis is possible with single cell suspensions from organs or from small amounts of blood. A broad range of studies require the analysis of cytotoxic T cell responses (vaccination and cancer immunotherapy studies).

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