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JoVE Journal
Immunology and Infection

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Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
 

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Article DOI: 10.3791/58680-v 08:10 min November 28th, 2018
November 28th, 2018

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Summary

Here, we present a protocol for the ex vivo qualitative detection of antigen-specific CD8+ T cells. Analysis is possible with single cell suspensions from organs or from small amounts of blood. A broad range of studies require the analysis of cytotoxic T cell responses (vaccination and cancer immunotherapy studies).

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Simultaneous Quantification Anti-vector-specific CD8+ T Cells Anti-transgene-specific CD8+ T Cells MHC I Tetramer Staining Viral Vector Vaccination T-cell Immunology Viral Vector Vaccines Small Blood Sample Repeated Measurements CTL Detection Jasmine Rinnofner Annika Rossler Zoltan Banki Mouse Restraint Blood Collection Splenocyte Isolation Cell Strainer Erythrocyte Lysis Cell Counting Sample Concentration Facs Tube Preparation Organ Suspension Transfer Supernatant Discard
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