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Användning av enda kedja MHC-teknik för att undersöka Co-agonism i mänskliga CD8 + T cellaktivering
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1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Emerging Infectious Diseases Program,Duke-NUS Graduate Medical School, 4Singapore Immunology Network,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS),National University of Singapore, 6Department of Immunology, Wright-Fleming Institute,Imperial College London
Chapters
- 00:04Title
- 01:19Plating CHO Cells as Antigen Presenting Cells
- 04:03T Cell Activation Assay
- 07:52CHO Cell MHC Class I Staining
- 09:06Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
- 10:46Conclusion
Det här protokollet beskriver användningen av enda kedja MHC klass I komplex att undersöka molekylära interaktioner i mänskliga CD8 + T cellaktivering: generation av bakåtkompilerade antigenpresenterande celler som uttrycker enda kedja konstruerar, kultur av mänskliga CD8 + T cell klon och T-cells aktivering experiment.
