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Brug af enkelt kæde MHC teknologi at undersøge Co-agonisme i menneskelige CD8 + T-celle aktivering
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Brug af enkelt kæde MHC teknologi at undersøge Co-agonisme i menneskelige CD8 + T-celle aktivering

DOI:
10.3791/59126-v

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12:09 min

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February 28, 2019

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, , , , , , , , , ,
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 2Immunology Programme, Life Sciences Institute,National University of Singapore, 3Emerging Infectious Diseases Program,Duke-NUS Graduate Medical School, 4Singapore Immunology Network,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering (NGS),National University of Singapore, 6Department of Immunology, Wright-Fleming Institute,Imperial College London

Chapters

  • 00:04Title
  • 01:19Plating CHO Cells as Antigen Presenting Cells
  • 04:03T Cell Activation Assay
  • 07:52CHO Cell MHC Class I Staining
  • 09:06Results: Investigation of Co-agonism in Human CD8+ T Cell Activation
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver brugen af enkelt kæde MHC klasse I komplekser til at undersøge molekylære interaktioner i menneskelige CD8 + T celle aktivering: generation af manipuleret antigen præsentere celler, der udtrykker enkelt kæde konstruktioner, kultur menneskelige CD8 + T celle klon og T-celle aktivering eksperimenter.

Tags

Single Chain MHCCo-agonismHuman CD8+ T Cell ActivationNonstimulatory Peptide MHCAgonist Peptide MHCHepatitis B VirusHIV GagTetracycline-inducible ExpressionCHO CellsT Cell Activation ExperimentTrypsinization

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