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一种在裂变酵母中的深度固序辅助自发抑制屏
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast
Schizosaccharomyces pombe
Please note that all translations are automatically generated.
Click here for the English version.
一种在裂变酵母中的深度固序辅助自发抑制屏
DOI:
10.3791/59133-v
•
07:55 min
•
March 07, 2019
•
Bahjat F. Marayati
2
,
James B. Pease
,
Ke Zhang
2
1
Department of Biology
,
Wake Forest University
,
2
Center for Molecular Communication and Signaling
,
Wake Forest University
Chapters
00:04
Title
00:32
Plate Reader Assay
03:13
Selection of Suppressor Colonies and Confirmation of Phenotype
05:27
Results: Slow-growing Mutants Show Phenotypic Recovery and Genetic Confirmation of Identified Suppressor Mutations Verifies the Heritability of the Recovery Phenotype
06:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个简单的抑制屏幕协议在裂变酵母。这种方法是有效的, 无突变, 并选择性的突变, 经常发生在一个单一的基因组位点。 该协议适用于分离抑制剂, 以减轻由突变或药物引起的液体培养中的生长缺陷。
Tags
Fission Yeast
Schizosaccharomyces Pombe
Suppressor Screen
Deep Sequencing
Spontaneous Mutation
Growth Defect
Optical Density
Plate Reader
Automated Dilution
Kinetic Growth Curve
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