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Environment
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環境試料からの担鉄細胞高スループット スクリーニング: 植物細胞、一括土壌および根圏土壌
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JoVE Journal
Environment
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
Please note that all translations are automatically generated.
Click here for the English version.
環境試料からの担鉄細胞高スループット スクリーニング: 植物細胞、一括土壌および根圏土壌
DOI:
10.3791/59137-v
•
12:36 min
•
February 09, 2019
•
Ricky W. Lewis*
1
,
Anjuman A. Islam*
1
,
Christine Jade Dilla-Ermita
,
Scot H. Hulbert
,
Tarah S. Sullivan
1
Department of Crop and Soil Sciences
,
Washington State University
,
2
Department of Plant Pathology
,
Washington State University
Chapters
00:04
Title
01:01
Preparation of Chrome Azurol S (CAS)-Fe-Agar Medium
03:35
Pyoverdine/EDTA Standard Preparation
05:04
Collection of Environmental Samples: Soil and Plant Tissues
06:38
Preparation of Siderophore Enrichment Cultures and CAS-Fe Siderophore Production Assay
08:11
CAS-Fe Agar Assays for Detection of Siderophore Production in Environmental Samples
09:34
Results: Pyoverdine Equivalents of Siderophore Enrichment Cultures
11:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
担鉄細胞の潜在的な地上システムで微量栄養素のバイオアベイラビリティと売り上げ高に貢献環境試料の急速なスクリーニングのためのプロトコルを提案します。
Tags
Siderophore Screening
High-throughput
Environmental Samples
Bulk Soils
Rhizosphere Soils
Iron Cycling
Microbial Community
Agriculture Productivity
Farm Management Practices
Soil Health
CAS Assay
HDTMA
CAS Solution
PIPES Buffer Solution
Agarose
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