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JoVE Journal Neuroscience
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A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Article DOI: 10.3791/59220-v • 07:52 min • April 9th, 2019
April 9th, 2019 •
*1, *1, 1, 2, 2, 3, 1
1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS
* These authors contributed equally

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Summary
Automatic Translation

Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells.

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Human Blood-brain Interface Model Barrier Crossings Pathogens Medicines Brain Interactions Neuro System Microbes Infected Brain Pain Tumor BBB Model Mini Brain Florian Bakao PhD Student Polyester Membrane Culture Insert Device Endothelial Cell Medium Confluency Poly-D-lysine Laminin Fetal Bovine Serum (FBS) Human Neurons Astrocytes CHME Clone Five Cells
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