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在 Vivo 三维双光子显微镜中,利用玻璃微移器研究本地 ATP 喷射的血管反应
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
Please note that all translations are automatically generated.
Click here for the English version.
在 Vivo 三维双光子显微镜中,利用玻璃微移器研究本地 ATP 喷射的血管反应
DOI:
10.3791/59286-v
•
10:54 min
•
June 07, 2019
•
Changsi Cai
,
Stefan A. Zambach
,
Jonas C. Fordsmann
,
Micael Lønstrup
,
Kirsten J. Thomsen
,
Aske G. K. Jensen
,
Martin Lauritzen
2
1
Department of Neuroscience, Faculty of Medicine and Health Science
,
University of Copenhagen
,
2
Department of Clinical Neurophysiology
,
Rigshospitalet
Chapters
00:04
Title
00:57
Surgical Procedure: Tracheotomy
01:55
Surgical Procedure: Catheter Insertion
02:59
Surgical Procedure: Craniotomy
05:29
Two-photon Imaging
06:32
Insertion of the Glass Micro-pipette
09:11
Results: ATP Puffing by Micro-pipette Induces Vessel Dilation, followed by Constriction
10:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们使用玻璃微移液器和快速的双光子超堆成像方法提供优化的局部弹出程序,从而能够精确测量毛细管直径变化,并在三维中调查其调节。
Tags
Three-dimensional
Two-photon Microscopy
Conducted Vascular Responses
ATP Ejection
Glass Micro-pipette
Cerebral Capillaries
Cerebral Blood Flow Regulation
Astrocytic Calcium Responses
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