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Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

DOI:
10.3791/58788-v

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07:27 min

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January 31, 2019

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, , , , ,
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France

Chapters

  • 00:04Title
  • 00:42Dissection Area Preparation
  • 01:51Brain Slicing
  • 03:112-photon Live Imaging
  • 04:12Compound Application
  • 06:14Results: Microglial Response Analyses
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

Tags

Keywords: Two-photon ImagingMicroglial ProcessesATPSerotoninAcute Brain SlicesDirectional Motility3D Print InterfacePerfusion ChambersCholine ACSFVibrating SlicerInterface ChamberPeristaltic PumpRecording Chamber

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