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Sekvensiell Immunofluorescence og immunhistokjemi på Cryosectioned sebrafisk embryo
JoVE Journal
Biology
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JoVE Journal Biology
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Sekvensiell Immunofluorescence og immunhistokjemi på Cryosectioned sebrafisk embryo

DOI:
10.3791/59344-v

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09:20 min

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May 14, 2019

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1Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Chapters

  • 00:04Title
  • 01:06Embryo Embedding and Preparation of Cryosections
  • 02:31Immunofluorescence for pH3
  • 04:54Immunohistochemistry for Labeled Dextran
  • 07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
  • 08:21Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen demonstrerer sekvensiell immunofluorescence og immunhistokjemi på cryosections fra tidlig stadium sebrafisk embryo som muliggjør presise colocalization analyser i spesifikke celle populasjoner.

Tags

ImmunofluorescenceImmunohistochemistryCryosectionsZebrafish EmbryosCo-localizationAntibody CompatibilityCryosectioningCo-expressionEmbryo OrientationCryostatCharged Glass SlidesPBS WashHumid ChamberBarrier Pen

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