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冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

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冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学

DOI:

10.3791/59344-v

•

09:20 min

•

May 14, 2019

•

Jordan L. Ferguson*, Heather R. Shive*

¹Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Chapters

00:04Title
01:06Embryo Embedding and Preparation of Cryosections
02:31Immunofluorescence for pH3
04:54Immunohistochemistry for Labeled Dextran
07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
08:21Conclusion

Summary

Automatic Translation

该方案演示了早期斑马鱼胚胎的冷冻节的连续免疫荧光和免疫组织化学,从而对特定细胞群进行精确的共定位分析。

Tags

Immunofluorescence Immunohistochemistry Cryosections Zebrafish Embryos Co-localization Antibody Compatibility Cryosectioning Co-expression Embryo Orientation Cryostat Charged Glass Slides PBS Wash Humid Chamber Barrier Pen

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