Name: Brachial Artery Catheterization in Swine (Video) | JoVE Journal
Uploaded: 2019-03-30T13:00:00.000+00:00
Duration: 9 min 6 s
Description: Learn brachial artery catheterization in swine for accurate arterial blood pressure monitoring and blood gas sampling during surgery. This fast, reliable technique supports veterinary physiological assessment.

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5 minutes

This work was supported by the Canadian Institutes of Health Research and Canada Foundation for Innovation grant to V. K. Mushahwar. A. Toossi was supported by a Vanier Canada Graduate Scholarship, Alberta Innovates - Health Solutions Graduate Studentship and a Queen Elizabeth II Graduate Scholarship. V.K. Mushahwar is a Canada Research Chair in Functional Restoration. We would like to acknowledge Mr. J. Stack of Moss Street Productions for his help with the audio production and staff of the Surgical Medical Research Institute for their assistance with the procedures.

All procedures on experimental animals described in both the video and manuscript were approved by the Institutional Animal Care and Use Committee of the University of Alberta
1. Surgical anesthesia and surgical preparation of the pigs.
<ol>
	<li>Premedicate 50 kg Landrace-Yorkshire commercial pigs intramuscularly with the anesthetic drug cocktail containing ketamine hydrochloride (22 mg/kg,), xylazine hydrochloride (2.2 mg/kg) and glycopyrrolate hydrochloride (10 &#181;g/kg).</li>
	<li>Set up all equipment involved with monitoring clinical parameters at the end of the table near the pig&#39;s head. Ensure the equipment will not restrict access to the pig. Produce accurate arterial blood pressure measurement by placing the pressure transducer in a horizontal plane, level with the heart.</li>
	<li>Anesthetize the pigs with inhaled isoflurane gas (4%-5% isoflurane at 500-1000 mL/min O2) using a properly sized face mask. Visualize the vocal cords with a veterinary laryngoscope (17-25 cm long straight blade) and apply topical 10% lidocaine spay to the vocal cords to limit the risk of laryngospasm and airway obstruction.</li>
	<li>Intubate the pigs by inserting a cuffed endotracheal tube (9.0 mm internal diameter (ID)) through the vocal cords and maintain anesthesia with isoflurane gas (0.5%-3.0% isoflurane at 1000-2000 mL/min O2). Ventilate the pig on a mechanical ventilator (18-22 breaths/min) and ensure all expired anesthetic gas is scavenged and vented outside the surgical suite. Assess the level of anesthesia by jaw tone, and both pedal and palpebral reflex responses. 
	NOTE: Administer intravenous Lactated Ringer&#39;s Solution (LRS, 10-50 mL/kg/h; see step 1.6) to enhance hemodynamic function and reduce isoflurane gas anesthesia induced depression of cardiovascular output in swine26.</li>
	<li>Secure a pulse oximeter to the mucosal surface of the tongue with medical tape to monitor heart rate and the saturation of peripheral blood oxygenation (SpO2). Insert a temperature probe approximately 2-4 cm into the nasal cavity to monitor body temperature. Place the pigs on a heated table to maintain normal body temperature (38-40 &#176;C) during the surgical procedure.</li>
	<li>Ensure surgical sterility with proper tissue preparation.
	<ol>
		<li>Clean the external surface of the ear to prepare for venous catheterization with 10% povidone-iodine surgical scrub solution and allow the solution to air dry.</li>
		<li>With a 20 G, 1 inch intravenous catheter, catheterize a marginal ear vein to deliver either intravenous fluids (LRS; 10-50 mL/kg/h) or the addition of other anesthetic agents.</li>
		<li>Enhance pig anesthesia and analgesia if needed for invasive procedures with continuous intravenous remifentanil hydrochloride infusion (0.05-0.14 &#181;g/kg/min).</li>
	</ol>
	</li>
	<li>Place the pig in a lateral recumbent position and gently extend the front leg approximately 10-12 cm away from the shoulder. Clip the hair on the skin surface of the medial aspect of the brachium (upper forelimb). Landmark the distal brachial artery pulse by palpation. 
	NOTE: The landmarked location of the artery lies along an oblique plane with the brachium approximately 9 cm from the olecranon, and 5 cm from the flexor aspect of the elbow joint. The brachial artery travels proximally towards the caudal third of the scapula traversing the humerus.</li>
	<li>Similarly to step 1.6, ensure surgical sterility with proper tissue preparation. Clean the skin surface with 10% povidone-iodine surgical scrub solution and allow the solution to air dry. Drape the brachial artery catheterization site with four small disposable surgical drapes.</li>
</ol>
2. Tissue dissection and catheterization of the brachial artery
<ol>
	<li>Make a 6 cm skin incision with a scalpel blade to expose the underlying tissue. Bluntly dissect with Metzenbaum scissors along the medial surfaces of the biceps brachii, deepening the dissection, until the pulsating artery is identified.</li>
	<li>Use cotton swabs to gently tease away the adventitia from the brachial artery, median nerve and brachial vein; structures that are in close proximity and within the same fascial plane. Gentle dissection is required, importantly ensuring minimal injury to the median nerve during the procedure. The brachial artery lies approximately 2.0-2.5 cm underneath the skin and is medial to coracobrachialis and lateral to the tensor fasciae antibrachii and overlies a small segment of the medial head of the triceps muscle27,28. 
	NOTE: Place a retractor to keep the skin incision open, allowing easier access to the brachial artery. Place a second retractor (optional) to further assist in vessel exposure.</li>
	<li>Moisten all tissues with warm saline (37 &#176;C) for the entire dissection to retain better structural integrity and improved tissue handling during the procedure.</li>
	<li>Create a tunnel under the artery with blunted forceps, then pass three 2-0 polyglactin sutures underneath the artery. Intentionally, leave the ends of this suture relatively long (3-4 cm) to secure the catheter to the artery. Add a &#34;loose suture tie&#34; allowing for quick catheter fastening, to the first two sutures that are separated 1.0 cm from each other and are approximately 1.5-2.0 cm proximal to the third distal suture. Ligate the most distal suture first to occlude the artery.</li>
	<li>Insert a 22 G, 1 inch peripheral venous catheter into the artery and then advance the catheter (completely to the catheter hub) off the stylet into the vessel. Partially withdraw the stylet from the catheter to visualize arterial blood, ensuring proper vessel placement of the catheter. Then, firmly secure the catheter in the vessel by tying the middle suture. Remove the stylet and quickly cap the catheter to minimize bleeding.</li>
	<li>Flush the incision and catheter with warm saline (37 &#176;C). Tie the most proximal suture and importantly ensure that the distal suture is tightly secured around the catheter hub as this improves catheter stability and reduces accidental slippage of the catheter from the artery (i.e., during pig repositioning). 
	NOTE: If the initial placement of the catheter into the artery fails, or the vessel is injured, reinsert the catheter into the artery at a position approximately 0.25 cm proximal to the initial catheter insertion site.</li>
	<li>Quickly attach the LRS filled intravenous extension line with the connected arterial pressure transducer to the catheter, and then lavage the surgical site with warm saline (37 &#176;C), keeping tissues moist, and clean any blood that spilled into the surrounding tissue. Flush the catheter with saline to ensure catheter patency and prevent blood clots from forming along the catheter wall. 
	NOTE: Check for transducer arterial blood pressure line failures (i.e., leaks), establish transducer baseline by zeroing arterial pressure monitor measurements, and ensure proper arterial blood pressure wave formations.</li>
	<li>Ensure continued catheter patency by maintaining the flush port of the extension line pressurized above 250 mmHg, with a pressure infuser bag delivering 3-5 mL/min LRS.
	<ol>
		<li>Optional: Place either two 2-0 polypropylene or two 2-0 polyglactin sutures around the catheter hub or intravenous extension line hub to further improve catheter stability within the artery.</li>
	</ol>
	</li>
</ol>
3. Tissue closure and body positioning
<ol>
	<li>Close the muscle layers with a simple continuous suture pattern with a 2-0 polyglactin suture on a cutting or tapered needle and close the skin in a simple interrupted suture pattern with a 2-0 polypropylene suture on a cutting needle. 
	NOTE: Interchangeably, 2-0 polyglactin or 2-0 polypropylene sutures can be used to close muscle and skin.</li>
	<li>Place the pig in ventral recumbency by rotating the abdomen of a lateral recumbent pig toward the surgical table. A left sided lateral recumbent pig is rotated in a clockwise direction, while a right sided lateral recumbent pig is rotated in a counter clockwise direction.</li>
	<li>Place the catheterized forelimb at a 40&#176; angle to the midline of the vertebral column of the pig. This forelimb positioning generates the best arterial blood flow and the most accurate arterial blood pressure measurements.</li>
</ol>
4. Monitoring clinical parameters
<ol>
	<li>Measure hemodynamic and respiratory parameters as well as temperature throughout the entire anesthetic and surgical procedure using proper monitoring equipment.</li>
</ol>

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</ol>

The authors have nothing to disclose.

Arterial catheterization to measure arterial blood pressures and collect blood samples for arterial blood gas measurements has been established in a wide range of animal species5,6,7,8,9,10,11,12,13,<sup class="...

Surgical intervention is used in experimental research to develop animal models that enhance scientific development. The scientific literature is filled with examples of novel surgical animal models1,2,3. Surgical procedures are a complex process involving not only the manipulation of anatomical structures but also complicated physiological interactions with various drugs required for anesthesia and analgesia. This interplay can induce major changes in physiological processes within the animal and as such requires vigilant monitoring of the animal4. Clinically successful surgical outcomes have been associated with measurements of arterial blood gases and arterial blood pressures5. These clinical parameters require the ability to measure arterial blood pressure and collect arterial blood effectively, which in turn requires the successful catheterization of an artery6,7.
Arterial catheterization to collect arterial blood and measure pressure has been used in various animal species5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21 and in animals at different ages of development19,20,21 and has been directed at both recovery (clinical and diagnostic) procedures4,5,6,7,8 and non-recovery (experimental) procedures14,15,16,17,18. Moreover, the ease of arterial access and the location of the artery in the context of the surgical procedure are also important considerations when choosing an artery for arterial blood measurements. For example, the median caudal artery in dogs and the facial artery in horses, as well as the pedal artery in both dogs and horses, are used for diagnostic measurement and monitoring during recovery procedures6,7,8. In contrast, the carotid and femoral arteries are often catheterized in swine for either non-recovery or long-term catheter implantation experiments14,15,18.
In swine, arterial catheterization to measure either arterial blood pressure or collect arterial blood has routinely employed either the carotid, femoral, medial saphenous, or auricular arteries22,23. For specialized non-routine procedures, other more unusual arteries have been used, including the subclavian and iliac arteries, to measure the brachial artery anatomical tortuosity17 and image the aorta16, respectively. Regardless of which artery is chosen for catheterization, each artery has inherent advantages and disadvantages for its use. For instance, the auricular artery is anatomically easy to access, but its use may be limited to its close proximity to the marginal ear veins11,12. In comparison, the carotid artery is relatively large and robust24, but it lies deep within the jugular furrow and requires substantive tissue dissection25. As such, identifying another artery that could be catheterized to measure arterial pressure and collect arterial blood is warranted. This video and manuscript describe in detail the catheterization of the distal brachial artery in swine, a technique that could be applied to non-recovery procedures. Notably, the pig brachial artery catheterization was used to measure arterial blood pressures and arterial blood gas parameters during a lumbar spine surgery with hind limb measurements (the data from this part of the surgery is not presented).

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brachial artery catheterization in swine

Brachial Artery Catheterization in Swine

The video describes in detail the catheterization of the distal brachial artery in swine. This technique enables researchers to measure arterial blood pressure continuously and collect arterial blood samples to assess arterial blood gas measurements. Arterial blood pressures and arterial blood gases are important physiological parameters to monitor during experimental procedures. In swine, four common methods of arterial catheterization have been described, including catheterization of the carotid, femoral, auricular, and medial saphenous arteries. Each of these techniques have advantages, such as ease of access for the auricular artery, and disadvantages that include deep tissue dissection for carotid artery catheterization. The described alternative method of arterial catheterization in swine, the catheterization of the distal aspect of the brachial artery, is a rapid procedure that requires relatively minimal tissue dissection and provides information that is in line with data collected from other arterial catheterization sites. The procedure uses a medial approach along an oblique plane of the lower brachium, positioned between the olecranon and the flexor aspect of the elbow joint, and this approach allows researchers the major advantage of unimpeded freedom for procedures that involve the caudoventral, caudodorsal back, or hind limbs of the pig. Due to the location of the upper forelimb of the catheterized vessel and potential challenges of effective homeostasis following catheter removal from the artery, this technique may be limited to non-recovery procedures.

Brachial artery catheterization allows for continuous monitoring of arterial blood pressure and intermittent sampling of arterial blood during extended surgical procedures in swine. Measured parameters were collected from seven 50 kg Landrace-Yorkshire commercial pigs as described. The total time required to catheterize the brachial artery was 35.2 ± 4.4 min from the initial artery landmarking to final surgical incision closure (Figure 1). The arterial pressu...

The video describes in detail the catheterization of the distal brachial artery in swine. This procedure accurately measures arterial blood pressure and is a simple and fast method to collect samples for arterial blood gas measurements.

The video describes in detail the catheterization of the distal brachial artery in swine. This technique enables researchers to measure arterial blood ...

Research

JoVE Journal

Medicine

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Seldinger Technique Based Pulmonary Artery Catheterization: A Procedure to Insert Pulmonary Artery Catheter Through the Jugular Vein in a Porcine Model

Source:Rui Cerqueira1,2, Liliana Costa1, André Leite-Moreira1, Adelino Leite-Moreira1,2, André Lourenço1, Pedro Mendes-Ferreira11Department of Surgery and Physiology, Faculty of Medicine of the University of Porto.2Department of Cardiothoracic Surgery, Hospital Universitário de São João.The video demonstrates a procedure for obtaining vascular access in an anesthetized pig using ultrasound guidance. After disinfecting the site and administering local anesthesia, an ultrasound probe is used to...

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A reliable and reproducible approach for the insertion and maintenance of a tunneled Hickman catheter for long-term vascular access in swine is described. Placement of a central venous catheter allows for convenient daily sampling of whole blood from awake animals and intravenous administration of medication and fluids.

Hickman Catheter Use for Long-Term Vascular Access in a Preclinical Swine Model

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Learn brachial artery catheterization in swine for accurate arterial blood pressure monitoring and blood gas sampling during surgery. This fast, reliable technique supports veterinary physiological assessment.

Brachial Artery Catheterization in Swine

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