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Journal / Immunology and Infection / 用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理…
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Journal (Immunology and Infection)
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Journal (Immunology and Infection)
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
 
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用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理

Article DOI: 10.3791/59427-v • 07:21 min • June 23rd, 2019
June 23rd, 2019 •
*1,2, *1,2,3, 1,2, 2, 5, 5,6, 1, 1,3,4, 2,3,6
1Research Institute McGill University Health Centre, 2Department of Biological Sciences, Université de Québec à Montréal, 3Department of Microbiology & Immunology, McGill University, 4Department of Medicine, Division of Infectious Diseases, McGill University, 5Centre de Recherche du Centre Hospitalier de l'Université de Montréal, 6Département de microbiologie, infectiologie et immunologie, Université de Montréal
* These authors contributed equally

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Summary
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Click here for the English version.

我们描述了一种处理支气管性肠液的方法,以及通过抗逆转录病毒疗法对慢性艾滋病毒感染者进行匹配的外周血,以评估肺艾滋病毒储存。这些方法导致获得高度纯的CD4 T细胞和海泡巨噬细胞,随后可用于免疫分型和HIV DNA/RNA定量通过超敏感聚合酶链反应。

Tags

撤回,第148期,支气管性洗漱室(BAL),海螺性巨噬细胞,免疫分泌,CD4 T细胞,HIVRNA,HIV储存体,骨髓细胞,抗逆转录病毒疗法(ART),白细胞巨噬细胞(AM)
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