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Immunology and Infection
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移植物抗宿主病的诱导和<em在体内</em> T细胞监测中的应用MHC匹配的小鼠模型
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Induction of Graft-versus-host Disease and
In Vivo
T Cell Monitoring Using an MHC-matched Murine Model
Please note that all translations are automatically generated.
Click here for the English version.
移植物抗宿主病的诱导和<em在体内</em> T细胞监测中的应用MHC匹配的小鼠模型
DOI:
10.3791/3697-v
•
10:29 min
•
August 29, 2012
•
Bryan A. Anthony
,
Gregg A. Hadley
1
Department of Surgery
,
The Ohio State University Medical Center
Chapters
00:05
Title
01:10
Lethal Irradiation and Splenocyte Preparation
03:12
Bone Marrow Preparation and Removal
05:16
CD3 Depletion
06:08
L2G85.B6 (LUX+) CD8+ T Cell Purification and Injection Preparation
07:48
Bioluminescent Imaging
08:12
Results
10:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
小鼠骨髓移植是一种被广泛使用的技术,研究在人类移植物抗宿主病的免疫机制。监测能力的T细胞贩运模式<em在体内</em>允许在移植物抗宿主病的发展和延续的T细胞反应进行详细的分析。
Tags
Graft-versus-host Disease
Bone Marrow Transplant
Hematological Deficiencies
Alloreactive T Cells
Bioluminescent Imaging
T Cell Trafficking
L2G85.B6 Transgenic Mice
Luciferase
Specific T Cell Subsets
GVHD Promotion
Time Points
Xenogen IVIS CCD Camera
Living Image Software
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